Congratulations to Dr. Cecilia Lo and Dr. Michael Tsang on their recently funded NIH administrative supplement, "Assaying Heterotaxy Patient Genes in a Cilia Motility and Left-Right Patterning". This project will examine whether expression of the RCV can rescue the HTX phenotype elicited by MO gene knockdown in the zebrafish embryo. Also, it will establish genotype-phenotype correlation in ciliary motion defects and develop software for quantitative classification of ciliary motion defects using a computational approach with computer vision and machine learning algorithms for visual pattern recognition. Using this software, we will determine whether different RCVs are associated with different ciliary motion defects. This will provide insights into structure-function relationships in the regulation of cilia motility.